آمار
| تعداد نشریات | 50 |
| تعداد شماره‌ها | 2,211 |
| تعداد مقالات | 20,279 |
| تعداد مشاهده مقاله | 24,205,009 |
| تعداد دریافت فایل اصل مقاله | 22,094,154 |
تاثیر ذرات معلق بر میزان بیان ژن های گاتا 4 گاتا 6 و آکواپورین 9 در بیضه موش صحرایی | ||
| پژوهش‌هایی در کشت سلول و بافت کاریوتیک | ||
| دوره 3، شماره 1، خرداد 1401، صفحه 29-39 اصل مقاله (609.55 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.30495/rkctc.2023.74786.1054 | ||
| نویسندگان | ||
الناز نوشادی راد 1؛ کاظم پریور  2؛ سعید متصدی رزندی 3؛ پژمان مرتضوی 4؛ بتول قربانی یکتا 5
| ||
| 1گروه زیست شناسی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران ایران | ||
| 2گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3گروه مهندسی بهداشت محیط دانشکده بهداشت و ایمنی عمومی دانشگاه علوم پزشکی شهید بهشتی تهران ایران | ||
| 4گروه پاتوبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 5گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| Introduction and Aim: Particulate matter (PM) is one of the major components of air pollutants and is composed of a mixture of liquid droplets or solid particles which are suspended in the air with a diameter of 2.5µm (PM2.5) or less. In this study, the co-exposure effect of ambient fine particulate matter (PM2.5) and gaseous pollutants was assessed on the expression of aquaporin-9, GATA-4, and GATA-6 in testicles of male rats at the gene and protein levels. Materials and Methods: In this study, three experimental groups were assigned; the first group was exposed to gaseous pollutants along with PM2.5 and was called the TM group; the second group included rats exposed to only gaseous pollutants and was called the TJ group. The control group received clean air without any pollutants or PMs and was called the TC group. The exposure time was five hours per day which started from 9:00 A.M to 14:00 P.M for four days per week. The process of time exposure lasted six months. The levels of SO2, NO2, O3, and PM2.5 were routinely measured. The UV fluorescence method was utilized for the measurement of SO2, NO2, and O3, while the beta- attenuation technique was employed for the analysis of PM2.5. Results: The results demonstrated that the concentration of PM2.5 in the exposed groups was significantly higher than the level recommended by the WHO, after six months, whereas the levels of SO2, NO2, and O3 were significantly lower than the recommended levels. The order of increment in the levels of metals in PM2.5 was Al> Ca>Mn>Cu> Cd> Na > Fe > Cr> Ni> Mn > Pb. Conclusion: According to our findings, it is concluded that exposure to PM2.5 could cause male infertility problems and must be considered a health concern for males. | ||
| کلیدواژه‌ها | ||
| PM2.5 exposure؛ GATA-4؛ GATA-6؛ Aquaporin-9 | ||
| مراجع | ||
|
Arena, S., Arena, F., Maisano, D., Di Benedetto, V., Romeo, C. and Nicòtina, P. 2011. Aquaporin‐9 immunohistochemistry in varicocele testes as a consequence of hypoxia in the sperm production site. Andrologia, 43(1): 34-37.
Arima, H., Yamamoto, N., Sobue, K., Umenishi, F., Tada, T., Katsuya, H. and Asai, K. 2003. Hyperosmolar mannitol stimulates expression of aquaporins 4 and 9 through a p38 mitogen-activated protein kinase-dependent pathway in rat astrocytes. Journal of Biological Chemistry, 278(45): 44525-44534.
Cao, X.-n., Shen, L.-j., Yan, C., Zhou, Y., Xiong, G., Wang, Y.-c., Liu, Y., Liu, B., Tang, X.-l. and Guo, M. 2017. Urban fine particulate matter exposure causes male reproductive injury through destroying blood-testis barrier (btb) integrity. Toxicology letters, 266: 1-12.
Carré, J., Gatimel, N., Moreau, J., Parinaud, J. and Léandri, R. 2017. Does air pollution play a role in infertility?: A systematic review. Environmental Health, 16: 1-16.
Dejakam, A., Dejakam, A. and Hekmat, A. 2020. Evaluating of bdnf expression in blood cells of opium recovering patients with a new treatment method: A molecular marker. Research in Cell and Tissue, 1(1): 16-25.
Elkjær, M.-L., Vajda, Z., Nejsum, L., Kwon, T.-H., Jensen, U., Amiry-Moghaddam, M., Frøkiaer, J. and Nielsen, S. 2000. Immunolocalization of aqp9 in liver, epididymis, testis, spleen, and brain. Biochemical and biophysical research communications, 276(3): 1118-1128.
Haghighatfard, A., Andalib, S., Amini Faskhodi, M., Sadeghi, S., Ghaderi, A.H., Moradkhani, S., Rostampour, J., Tabrizi, Z., Mahmoodi, A. and Karimi, T. 2018. Gene expression study of mitochondrial complex i in schizophrenia and paranoid personality disorder. The World Journal of Biological Psychiatry, 19(sup3): S133-S146.
Hansen, C., Luben, T.J., Sacks, J.D., Olshan, A., Jeffay, S., Strader, L. and Perreault, S.D. 2010. The effect of ambient air pollution on sperm quality. Environmental health perspectives, 118(2): 203-209.
Heikinheimo, M., Ermolaeva, M., Bielinska, M., Rahman, N.A., Narita, N., Huhtaniemi, I.T., Tapanainen, J.S. and Wilson, D.B. 1997. Expression and hormonal regulation of transcription factors gata-4 and gata-6 in the mouse ovary. Endocrinology, 138(8): 3505-3514.
Hu, Y.-C., Okumura, L.M. and Page, D.C. 2013. Gata4 is required for formation of the genital ridge in mice. PLoS genetics, 9(7): e1003629.
Jurewicz, J., Radwan, M., Sobala, W., Polańska, K., Radwan, P., Jakubowski, L., Ulańska, A. and Hanke, W. 2015. The relationship between exposure to air pollution and sperm disomy. Environmental and molecular mutagenesis, 56(1): 50-59.
Karottki, D.G., Spilak, M., Frederiksen, M., Gunnarsen, L., Brauner, E.V., Kolarik, B., Andersen, Z.J., Sigsgaard, T., Barregard, L. and Strandberg, B. 2013. An indoor air filtration study in homes of elderly: Cardiovascular and respiratory effects of exposure to particulate matter. Environmental Health, 12: 1-10.
Ketola, I., Anttonen, M., Vaskivuo, T., Tapanainen, J.S., Toppari, J. and Heikinheimo, M. 2002. Developmental expression and spermatogenic stage specificity of transcription factors gata-1 and gata-4 and their cofactors fog-1 and fog-2 in the mouse testis. European Journal of Endocrinology, 147(3): 397-406.
Ketola, I., Pentikäinen, V., Vaskivuo, T., Ilvesmäki, V., Herva, R., Dunkel, L., Tapanainen, J.S., Toppari, J. and Heikinheimo, M. 2000. Expression of transcription factor gata-4 during human testicular development and disease. The Journal of Clinical Endocrinology & Metabolism, 85(10): 3925-3931.
Ketola, I., Toppari, J., Vaskivuo, T., Herva, R., Tapanainen, J.S. and Heikinheimo, M. 2003. Transcription factor gata-6, cell proliferation, apoptosis, and apoptosis-related proteins bcl-2 and bax in human fetal testis. The Journal of Clinical Endocrinology & Metabolism, 88(4): 1858-1865.
Li, R., Zhao, Y., Shi, J., Zhao, C., Xie, P., Huang, W., Yong, T. and Cai, Z. 2020. Effects of pm2. 5 exposure in utero on heart injury, histone acetylation and gata4 expression in offspring mice. Chemosphere, 256: 127133.
Li, X., Jin, L. and Kan, H. 2019. Air pollution: A global problem needs local fixes. Nature, 570(7762): 437-439.
Liu, X., Jin, X., Su, R. and Li, Z. 2017. The reproductive toxicology of male sd rats after pm2. 5 exposure mediated by the stimulation of endoplasmic reticulum stress. Chemosphere, 189: 547-555.
Madaniyazi, L., Li, S., Li, S. and Guo, Y. 2020. Candidate gene expression in response to low-level air pollution. Environment international, 140: 105610.
Manisalidis, I., Stavropoulou, E., Stavropoulos, A. and Bezirtzoglou, E. 2020. Environmental and health impacts of air pollution: A review. Frontiers in public health, 8: 14.
Molkentin, J.D. 2000. The zinc finger-containing transcription factors gata-4,-5, and-6: Ubiquitously expressed regulators of tissue-specific gene expression. Journal of Biological Chemistry, 275(50): 38949-38952.
Morrisey, E.E., Tang, Z., Sigrist, K., Lu, M.M., Jiang, F., Ip, H.S. and Parmacek, M.S. 1998. Gata6 regulates hnf4 and is required for differentiation of visceral endoderm in the mouse embryo. Genes & development, 12(22): 3579-3590.
Pastor-Soler, N., Bagnis, C., Sabolic, I., Tyszkowski, R., McKee, M., Van Hoek, A., Breton, S. and Brown, D. 2001. Aquaporin 9 expression along the male reproductive tract. Biology of reproduction, 65(2): 384-393.
Rahmanian, N., Hekmat, A. and Hajebrahimi, Z. Effect of simulated microgravity condition on cells proliferation and myostatin expression in differentiated skeletal muscle cells (c2c12).
Raymond, C.S., Parker, E.D., Kettlewell, J.R., Brown, L.G., Page, D.C., Kusz, K., Jaruzelska, J., Reinberg, Y., Flejter, W.L. and Bardwell, V.J. 1999. A region of human chromosome 9p required for testis development contains two genes related to known sexual regulators. Human molecular genetics, 8(6): 989-996.
Raymond, C.S., Shamu, C.E., Shen, M.M., Seifert, K.J., Hirsch, B., Hodgkin, J. and Zarkower, D. 1998. Evidence for evolutionary conservation of sex-determining genes. Nature, 391(6668): 691-695.
Robert, N.M., Tremblay, J.J. and Viger, R.S. 2002. Friend of gata (fog)-1 and fog-2 differentially repress the gata-dependent activity of multiple gonadal promoters. Endocrinology, 143(10): 3963-3973.
Sicard, P., Khaniabadi, Y.O., Perez, S., Gualtieri, M. and De Marco, A. 2019. Effect of o 3, pm 10 and pm 2.5 on cardiovascular and respiratory diseases in cities of france, iran and italy. Environmental science and pollution research, 26: 32645-32665.
Sowlat, M.H., Naddafi, K., Yunesian, M., Jackson, P.L. and Shahsavani, A. 2012. Source apportionment of total suspended particulates in an arid area in southwestern iran using positive matrix factorization. Bulletin of environmental contamination and toxicology, 88: 735-740.
Tsukaguchi, H., Weremowicz, S., Morton, C.C. and Hediger, M.A. 1999. Functional and molecular characterization of the human neutral solute channel aquaporin-9. American Journal of Physiology-Renal Physiology, 277(5): F685-F696.
Wang, L., Luo, D., Liu, X., Zhu, J., Wang, F., Li, B. and Li, L. 2021. Effects of pm2. 5 exposure on reproductive system and its mechanisms. Chemosphere, 264: 128436.
Watanabe, N. and Oonuki, Y. 1999. Inhalation of diesel engine exhaust affects spermatogenesis in growing male rats. Environmental Health Perspectives, 107(7): 539-544.
Weagle, C.L., Snider, G., Li, C., Van Donkelaar, A., Philip, S., Bissonnette, P., Burke, J., Jackson, J., Latimer, R. and Stone, E. 2018. Global sources of fine particulate matter: Interpretation of pm2. 5 chemical composition observed by spartan using a global chemical transport model. Environmental science & technology, 52(20): 11670-11681.
Wei, Y., Cao, X.-N., Tang, X.-L., Shen, L.-J., Lin, T., He, D.-W., Wu, S.-D. and Wei, G.-H. 2018. Urban fine particulate matter (pm2. 5) exposure destroys blood–testis barrier (btb) integrity through excessive ros-mediated autophagy. Toxicology mechanisms and methods, 28(4): 302-319.
Wu, X., Pan, B., Liu, L., Zhao, W., Zhu, J., Huang, X. and Tian, J. 2019. In utero exposure to pm2. 5 during gestation caused adult cardiac hypertrophy through histone acetylation modification. Journal of Cellular Biochemistry, 120(3): 4375-4384.
Zarandi, S.M., Shahsavani, A., Khodagholi, F. and Fakhri, Y. 2019. Co-exposure to ambient pm2. 5 plus gaseous pollutants increases amyloid β1–42 accumulation in the hippocampus of male and female rats. Toxin reviews.
Zhang, Y., West, J.J., Mathur, R., Xing, J., Hogrefe, C., Roselle, S.J., Bash, J.O., Pleim, J.E., Gan, C.-M. and Wong, D.C. 2018. Long-term trends in the ambient pm 2.5-and o 3-related mortality burdens in the united states under emission reductions from 1990 to 2010. Atmospheric chemistry and physics, 18(20): 15003-15016.
Zhou, L., Su, X., Li, B., Chu, C., Sun, H., Zhang, N., Han, B., Li, C., Zou, B. and Niu, Y. 2019. Pm2. 5 exposure impairs sperm quality through testicular damage dependent on nalp3 inflammasome and mir-183/96/182 cluster targeting foxo1 in mouse. Ecotoxicology and Environmental Safety, 169: 551-563.
Zhou, N., Cui, Z., Yang, S., Han, X., Chen, G., Zhou, Z., Zhai, C., Ma, M., Li, L. and Cai, M. 2014. Air pollution and decreased semen quality: A comparative study of chongqing urban and rural areas. Environmental Pollution, 187: 145-152. | ||
|
آمار تعداد مشاهده مقاله: 40 تعداد دریافت فایل اصل مقاله: 32 |
||
