تعداد نشریات | 50 |
تعداد شمارهها | 2,232 |
تعداد مقالات | 20,476 |
تعداد مشاهده مقاله | 25,347,858 |
تعداد دریافت فایل اصل مقاله | 23,004,279 |
Metal ions binding study on human growth hormone by isothermal titration calorimetric method | ||
Journal of Physical & Theoretical Chemistry | ||
مقاله 2، دوره 2، شماره 4، اردیبهشت 2006، صفحه 13-26 اصل مقاله (476.35 K) | ||
نوع مقاله: Research Paper | ||
نویسندگان | ||
A.A. Saboury* ؛ MS. Atri* | ||
Not-mentioned | ||
چکیده | ||
The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for and , and also their molar enthalpies of binding (KJ/mol for and KJ/mol for ) are similar showing same thermodynamical properties for hGH upon interaction with both and . Thermodynamical properties for hGH upon interaction with both is completely different from those of and . The affinity of binding is the highest (), but the molar enthalpy of binding is the lowest () for . There is a set of four identical and independent binding sites for . The binding process for iron ions is more exothermic (KJ/mol) than other three metal ions and with a higher affinity () respect to and | ||
کلیدواژهها | ||
Isothermal titration calorimetry؛ Human growth hormone؛ Calcium؛ magnesium؛ Copper؛ Iron(III) | ||
آمار تعداد مشاهده مقاله: 825 تعداد دریافت فایل اصل مقاله: 404 |